Cryopreservation

Fertility Cryopreservation

Oocyte cryopreservation.

OBJECTIVE To review historical and contemporary advances in oocyte-cryopreservation techniques and outcomes. DESIGN Publications related to oocyte cryopreservation were identified through MEDLINE and other bibliographic databases. CONCLUSION(S) Oocyte cryopreservation can be used as an adjunct to conventional IVF and as an option for fertile women to electively cryopreserve their gametes. R...

Aldehyde-stabilized cryopreservation.

We describe here a new cryobiological and neurobiological technique, aldehyde-stabilized cryopreservation (ASC), which demonstrates the relevance and utility of advanced cryopreservation science for the neurobiological research community. ASC is a new brain-banking technique designed to facilitate neuroanatomic research such as connectomics research, and has the unique ability to combine stable...

Improving cryopreservation systems.

Cryopreservation of oocytes and embryos is a crucial step for widespread practical application of other techniques in domestic animal embryology. However, in contrast to the rapid development of procedures in the eighties and early nineties of the last century, new advancements with profound practical consequences have only been recently achieved. As a result of a long and controversial develop...

Microfluidics for cryopreservation.

Minimizing cell damage throughout the cryopreservation process is critical to enhance the overall outcome. Osmotic shock sustained during the loading and unloading of cryoprotectants (CPAs) is a major source of cell damage during the cryopreservation process. We introduce a microfluidic approach to minimize osmotic shock to cells during cryopreservation. This approach allows us to control the l...